Bacteria thread the needle.
نویسندگان
چکیده
Scanning microscopes will be vital elements in the atom engineer's toolbox, and our understanding of their operation continues to advance rapidly. We can look forward to finding a sound basis not only for imaging and spectroscopy of single atoms, but also their manipulation and the creation of wholly novel nanostructures. (residues 35–139) binds to SicP in an extended , non-globular conformation in which its secondary structures (Ȋ-helices and ȋ-strands) remain intact (Fig. 1). The grooves and crevices in SicP that make intricate contact with the SptP fragment are probably the basis for type III chaperone specificity. The amphipathic helix in the chaperone was thought to be important in protein–protein interactions 7 , but it has no direct interaction with the effector and is not involved in chaperone dimerization. So it is likely to be a general structural feature of these chaperones. The protein's structure was crystallized as a crossed-over SicP–SptP 4:2 complex (Fig. 1). Luo et al. 8 have used light scattering to show that other type III chaperone–effector complexes exist in a 2:1 stoichiometry. In the crystal structure reported here, there is a potential domain swap involving helix 5. This domain swap may occur during the crystallization process and subsequently be stabilized by a disulphide bond that is observed in the crystal structure but not in the purified complex before crystallography. So, as the authors point out, it is possible that the biologically relevant complex has a 2:1 stoichiometry. Type III effector proteins have one or more functional domains. For a protein to travel through the secretion needle, each domain must physically be able to fit inside the cylindrical cavity of the channel. If the domain is wider than the secretion tube, it can't pass through it. On the evidence of its previously solved crystal structure, SptP has two domains. The diameter of the secretion channel has been estimated to be about 30 Å
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ورودعنوان ژورنال:
- Nature
دوره 414 6859 شماره
صفحات -
تاریخ انتشار 2001